Anti-gamma Tubulin antibody - Centrosome Marker (ab16504)
Key features and details
- Rabbit polyclonal to gamma Tubulin - Centrosome Marker
- Suitable for: ICC/IF, WB
- Reacts with: Human, Zebrafish
- Isotype: IgG
Overview
-
Product name
Anti-gamma Tubulin antibody - Centrosome Marker
See all gamma Tubulin primary antibodies -
Description
Rabbit polyclonal to gamma Tubulin - Centrosome Marker -
Host species
Rabbit -
Specificity
Does not react with alpha or beta tubulin. Immunogen sequence found in both gamma tubulin 1 and gamma tubulin 2. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Zebrafish
Predicted to work with: Mouse, Rat, Dog, Xenopus laevis
-
Immunogen
Synthetic peptide corresponding to Human gamma Tubulin aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab17097)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab16504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Abcam recommends using milk as the blocking agent.
Target
-
Function
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
-
Database links
- Entrez Gene: 403695 Dog
- Entrez Gene: 490962 Dog
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
- Entrez Gene: 103768 Mouse
- Entrez Gene: 252921 Rat
- Entrez Gene: 680991 Rat
see all -
Alternative names
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504)
ICC/IF image of ab16504 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16504, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
-
Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504)All lanes : Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Cell Lysate
Lane 3 : A431 Whole Cell Lysate
Lane 4 : HeLa Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml
Lane 5 : HeLa Nuclear Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml
Lane 6 : A431 Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDaLane 1 - 6 : gamma Tubulin antibody - Centrosome Marker (ab11317) at 1 ug/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Cell Lysate
Lane 3 : A431 Whole Cell Lysate
Lane 4 : HeLa Whole Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Lane 5 : HeLa Nuclear Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Lane 6 : A431 Whole Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Lysates at 20 ug.
Blocking peptide at 1 ug/ml.
Performed under reducing conditions.
Observed band size : 48kD -
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504)This image is courtesy of Darin McDonald, Cross Cancer InstituteSK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16504 (1/300). ab16504 staining is localized to the centrosome (red). The cells were counterstained with DAPI (blue). 100x magnification. The cells were blocked with 5% fetal bovine serum.
-
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504)This image is courtesy of Darin McDonald, Cross Cancer InstituteSK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16504. The antibody clearly labels the centrosome (red). The cells were counterstained with DAPI (blue). The cells were blocked in 5% BSA.
-
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504)ICC/IF image of ab16504 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16504, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The cause of the background staining is uncertain, although a cytokeratin exisits of a similar molecular weight and amino acid sequence to that of the immunogen used to raise the antibody. -
Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504)All lanes : Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) at 1 µg/ml
Lane 1 : Molecular Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish liver homogenate at 20 µg
Lane 4 : HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg
Secondary
Lane 1 : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Lanes 2-4 : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 5 minutes
Protocols
References (22)
ab16504 has been referenced in 22 publications.
- Ma Z et al. Primary cilia-dependent signaling is involved in regulating mesenchymal stem cell proliferation and pluripotency maintenance. J Mol Histol 51:241-250 (2020). PubMed: 32399704
- Ishikawa KI et al. p150glued deficiency impairs effective fusion between autophagosomes and lysosomes due to their redistribution to the cell periphery. Neurosci Lett 690:181-187 (2019). PubMed: 30366015
- Revinski DR et al. CDC20B is required for deuterosome-mediated centriole production in multiciliated cells. Nat Commun 9:4668 (2018). PubMed: 30405130
- Watanuki S et al. Characterization of centriole duplication in human epidermis, Bowen's disease, and squamous cell carcinoma. J Dermatol Sci 91:9-18 (2018). ICC/IF . PubMed: 29615326
- Scerbo P et al. Lineage commitment of embryonic cells involves MEK1-dependent clearance of pluripotency regulator Ventx2. Elife 6:N/A (2017). In situ hybridization ; Xenopus laevis . PubMed: 28654420
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504)
ICC/IF image of ab16504 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16504, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
-
Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504)All lanes : Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Cell Lysate
Lane 3 : A431 Whole Cell Lysate
Lane 4 : HeLa Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml
Lane 5 : HeLa Nuclear Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml
Lane 6 : A431 Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDaLane 1 - 6 : gamma Tubulin antibody - Centrosome Marker (ab11317) at 1 ug/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Cell Lysate
Lane 3 : A431 Whole Cell Lysate
Lane 4 : HeLa Whole Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Lane 5 : HeLa Nuclear Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Lane 6 : A431 Whole Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Lysates at 20 ug.
Blocking peptide at 1 ug/ml.
Performed under reducing conditions.
Observed band size : 48kD -
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) This image is courtesy of Darin McDonald, Cross Cancer Institute
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16504 (1/300). ab16504 staining is localized to the centrosome (red). The cells were counterstained with DAPI (blue). 100x magnification. The cells were blocked with 5% fetal bovine serum.
-
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) This image is courtesy of Darin McDonald, Cross Cancer Institute
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16504. The antibody clearly labels the centrosome (red). The cells were counterstained with DAPI (blue). The cells were blocked in 5% BSA.
-
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504)ICC/IF image of ab16504 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16504, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The cause of the background staining is uncertain, although a cytokeratin exisits of a similar molecular weight and amino acid sequence to that of the immunogen used to raise the antibody. -
Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab16504)All lanes : Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) at 1 µg/ml
Lane 1 : Molecular Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish liver homogenate at 20 µg
Lane 4 : HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg
Secondary
Lane 1 : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Lanes 2-4 : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 5 minutes
