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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules Tubulin

Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)

Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13477(B)] to TUBA4A - BSA and Azide free
  • Suitable for: IHC-P, ICC, IP, WB, Flow Cyt
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free
    See all TUBA4A primary antibodies
  • Description

    Rabbit monoclonal [EPR13477(B)] to TUBA4A - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249984 is the carrier-free version of ab177479. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249984 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13477(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin

Images

  • Western blot - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    Western blot - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    All lanes : Anti-TUBA4A antibody [EPR13477(B)] (ab177479) at 1/1000 dilution

    Lane 1 : Molt-4 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 50 kDa



    This data was developed using ab177479, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    This data was developed using ab177479, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1X PBS (negative control) using ab177479, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    This data was developed using ab177479, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TUBA4A with ab177479 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    This data was developed using ab177479, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TUBA4A with ab177479 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    Immunocytochemistry - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    This data was developed using ab177479, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of A431 cells labeling TUBA4A with ab177479 at 1/100 dilution (red). DAPI nuclear staining (blue).
  • Flow Cytometry - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    Flow Cytometry - Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    This data was developed using ab177479, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized K562 cells labeling TUBA4A with ab177479 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
  • Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)
    Anti-TUBA4A antibody [EPR13477(B)] - BSA and Azide free (ab249984)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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