Anti-Bcl-2 antibody (ab196495)
Key features and details
- Rabbit polyclonal to Bcl-2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Bcl-2 antibody
See all Bcl-2 primary antibodies -
Description
Rabbit polyclonal to Bcl-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseRatHumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Bcl-2. (Near N terminal).
Database link: P10415 -
Positive control
- ICC/IF: A549 cells. IHC-P: Rat kidney and lung tissues; Mouse lung tissue; Human liver cancer tissue; Breast cancer tissue. WB: HL-60 and BT474 cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bcl-2 antibody (ab196495)All lanes : Anti-Bcl-2 antibody (ab196495) at 1/500 dilution
Lane 1 : HL-60 cell extract
Lane 2 : BT474 cell extract
Predicted band size: 26 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (ab196495)Immunofluorescent analysis of A549 (human lung carcinoma cell line) cells labeling Bcl-2 with ab196495 at 1/50 dilution. Blue: DAPI for nuclear staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (ab196495)Immunohistochemical analysis of paraffin-embedded breast cancer tissue labeling Bcl-2 with ab196495 at 1/50 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (ab196495)Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling Bcl-2 with ab196495 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (ab196495)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Bcl-2 with ab196495 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (ab196495)Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Bcl-2 with ab196495 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (ab196495)Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Bcl-2 with ab196495 at 1/100 dilution.
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Western blot - Anti-Bcl-2 antibody (ab196495) Singh, A. et al. Imprinting of cerebral cytochrome P450s in offsprings prenatally exposed to cypermethrin augments toxicity on rechallenge. Sci. Rep. 6, 37426; doi: 10.1038/srep37426 (2016).All lanes : Anti-Bcl-2 antibody (ab196495)
Lane 1 : Total protein isolated from brain of rat offspring raised on control mothers
Lane 2 : Total protein isolated from brain of rat offspring exposed prenatally to 5 mg/kg of cypermethrin
Lane 3 : Total protein isolated from brain of rat offspring exposed post natally to 10 mg/kg of cypermethrin
Lane 4 : Total protein isolated from brain of rat offspring exposed prenatally to 5 mg/kg of cypermethrin and subsequently rechallenged with cypermethrin (10 mg/kg) at adulthood
Lysates/proteins at 50 µg per lane.
Predicted band size: 26 kDa
