Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
![Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)](https://www.abcam.com/ps/products/173/ab173831/Images/ab173831-197564-anti-gamma-tubulin-antibody-4d11-c-terminal-immunofluorescence.jpg "Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)")
Key features and details
- Mouse monoclonal [4D11] to gamma Tubulin - C-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-gamma Tubulin antibody [4D11] - C-terminal
See all gamma Tubulin primary antibodies -
Description
Mouse monoclonal [4D11] to gamma Tubulin - C-terminal -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment within Human gamma Tubulin aa 437-451 (C terminal). The exact sequence is proprietary.
Sequence:AATRPDYISWGTQEQ
Database link: P23258 -
Positive control
- Human breast carcinoma and colon tissues; HeLa and U2OS cells; HeLa, HepG2, U2OS and K562 cell lysates; mouse 3T3L1 and spleen lysates; monkey COS7 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4D11 -
Isotype
IgG2b -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
Immunofluorescence analysis of formalin-fixed, permeabilized A) HeLa cells and B) U2OS, labeling gamma Tubulin (green) using ab173831 at a 1/50 dilution. Cells were washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. F-Actin (red) was stained with DyLight 547 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
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![Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)](https://www.abcam.com/ps/products/173/ab173831/Images/ab173831-197561-anti-gamma-tubulin-antibody-4d11-c-terminal-western-blot.jpg)
Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)All lanes : Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U2OS cell lysate
Lane 4 : K562 cell lysate
Lane 5 : mouse 3T3L1 cell lysate
Lane 6 : mouse spleen lysate
Lane 7 : monkey COS7 cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-mouse-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 51 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)](https://www.abcam.com/ps/products/173/ab173831/Images/ab173831-197562-anti-gamma-tubulin-antibody-4d11-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)Immunohistochemical analysis of Human breast carcinoma tissue, labeling gamma Tubulin with ab173831 at a 1/20 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)](https://www.abcam.com/ps/products/173/ab173831/Images/ab173831-197563-anti-gamma-tubulin-antibody-4d11-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)Immunohistochemical analysis of Human colon tissue, labeling gamma Tubulin with ab173831 at a 1/200 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
