Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

Key features and details

  • Mouse monoclonal [4D11] to gamma Tubulin - C-terminal
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human, African green monkey
  • Isotype: IgG2b

Overview

  • Product name

    Anti-gamma Tubulin antibody [4D11] - C-terminal
    See all gamma Tubulin primary antibodies

  • Description

    Mouse monoclonal [4D11] to gamma Tubulin - C-terminal

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human gamma Tubulin aa 437-451 (C terminal). The exact sequence is proprietary.
    Sequence:

    AATRPDYISWGTQEQ


    Database link: P23258
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human breast carcinoma and colon tissues; HeLa and U2OS cells; HeLa, HepG2, U2OS and K562 cell lysates; mouse 3T3L1 and spleen lysates; monkey COS7 cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

    Immunofluorescence analysis of formalin-fixed, permeabilized A) HeLa cells and B) U2OS, labeling gamma Tubulin (green) using ab173831 at a 1/50 dilution. Cells were washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. F-Actin (red) was stained with DyLight 547 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

  • Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
    Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
    All lanes : Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : U2OS cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : mouse 3T3L1 cell lysate
    Lane 6 : mouse spleen lysate
    Lane 7 : monkey COS7 cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : goat anti-mouse-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

    Immunohistochemical analysis of Human breast carcinoma tissue, labeling gamma Tubulin with ab173831 at a 1/20 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

    Immunohistochemical analysis of Human colon tissue, labeling gamma Tubulin with ab173831 at a 1/200 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

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