Anti-CKAP1/TBCB antibody (ab237483)
Key features and details
- Rabbit polyclonal to CKAP1/TBCB
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CKAP1/TBCB antibody
See all CKAP1/TBCB primary antibodies -
Description
Rabbit polyclonal to CKAP1/TBCB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human CKAP1/TBCB aa 1 to the C-terminus.
Database link: Q99426-1 -
Positive control
- WB: HeLa, A431, A549 and Jurkat whole cell lysate; Mouse thymus tissue lysate. IHC-P: Human kidney tissue ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
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General notes
This product was previously labelled as CKAP1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human kidney tissue stained for CKAP1/TBCB with ab237483 at a 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-CKAP1/TBCB antibody (ab237483) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : Mouse thymus tissue lysate
Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
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HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for CKAP1/TBCB (Green) using ab237483 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
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CKAP1/TBCB was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate using 4 µg of ab237483.
Lane 1: Rabbit control IgG (1 µg) in HeLa whole cell lysate.
Lane 2: ab237483 (4 µg) in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (20 µg input).
For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at a 1/50,000 dilution.