Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-HSF1 antibody [EPR23673-128] - BSA and Azide free (ab275382)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23673-128] to HSF1 - BSA and Azide free
Suitable for: WB, Flow Cyt, ICC
Reacts with: Mouse, Rat

Product name

Anti-HSF1 antibody [EPR23673-128] - BSA and Azide free
See all HSF1 primary antibodies
Description

Rabbit monoclonal [EPR23673-128] to HSF1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, Flow Cyt, ICCmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse ovary and testis tissue lysates; Rat testis tissue lysate; NIH/3T3 and PC-12 whole cell lysates. ICC: NIH/3T3 and C2C12 cells. Flow cyt: NIH/3T3 cells.
General notes
ab275382 is the carrier-free version of ab242138. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275382 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23673-128
Isotype

IgG
Research areas

Western blot - Anti-HSF1 antibody [EPR23673-128] - BSA and Azide free (ab275382)

All lanes : Anti-HSF1 antibody [EPR23673-128] (ab242138) at 1/1000 dilution

Lane 1 : Mouse ovary tissue lysate
Lane 2 : Mouse testis tissue lysate
Lane 3 : Rat testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Lane 3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051 at 1/50000 dilution

Predicted band size: 57 kDa
Observed band size: 80 kDa
why is the actual band size different from the predicted?

This data was developed using ab242138, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10747023, 28194040, 27474884).

Exposure time: 3 minutes.
Flow Cytometry - Anti-HSF1 antibody [EPR23673-128] - BSA and Azide free (ab275382)

This data was developed using ab242138, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling HSF1 with ab242138 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry - Anti-HSF1 antibody [EPR23673-128] - BSA and Azide free (ab275382)

This data was developed using ab242138, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labelling HSF1 with ab242138 at 1/50 (9.4 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Western blot - Anti-HSF1 antibody [EPR23673-128] - BSA and Azide free (ab275382)

All lanes : Anti-HSF1 antibody [EPR23673-128] (ab242138) at 1/1000 dilution

Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?

This data was developed using ab242138, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Lysates were made freshly and used in WB test immediately to minimize protein degradation.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10747023, 28194040, 27474884).

Exposure times: Lane 1: 15 seconds; Lane 2: 37 seconds.
Immunocytochemistry - Anti-HSF1 antibody [EPR23673-128] - BSA and Azide free (ab275382)

This data was developed using ab242138, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling HSF1 with ab242138 at 1/50 (9.4 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in C2C12 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Anti-HSF1 antibody [EPR23673-128] - BSA and Azide free (ab275382)

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