Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)
![Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)](https://www.abcam.com/ps/products/238/ab238942/Images/ab238942-415234-anti-renilla-luciferase-antibody-epr17792-westernblot-293t-human.jpg "Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17792] to Renilla Luciferase - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, WB
Overview
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Product name
Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free
See all Renilla Luciferase primary antibodies -
Description
Rabbit monoclonal [EPR17792] to Renilla Luciferase - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Other species
Does not react with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Renilla Luciferase transfected HEK293T whole cell lysate. ICC/IF: Renilla Luciferase transfected HEK293T cells. Flow Cyt: Renilla Luciferase transfected HEK293T cells.
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General notes
Ab238942 is the carrier-free version of ab185926. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab238942 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17792 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)All lanes : Anti-Renilla Luciferase antibody [EPR17792] (ab185926) at 1/1000 dilution (Purified)
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with Renilla Luciferase expression vector, whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185926).
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![Flow Cytometry - Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)](https://www.abcam.com/ps/products/238/ab238942/Images/ab238942-2-ab185926273211185926FC1.jpg)
Flow Cytometry - Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) transfected with GFP tagged Renilla Luciferase cells labeled with purified ab185926 at 1/70 dilution (10µg/ml, Right panel) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185926).
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![Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)](https://www.abcam.com/ps/products/238/ab238942/Images/ab238942-317598-399.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185926 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Renilla Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab185926 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185936).
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![Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)](https://www.abcam.com/ps/products/238/ab238942/Images/ab238942-4-benefits-of-recombinant-antibodies.png)
Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)
