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Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17792] to Renilla Luciferase - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, WB

Overview

  • Product name

    Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free
    See all Renilla Luciferase primary antibodies

  • Description

    Rabbit monoclonal [EPR17792] to Renilla Luciferase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Other species
    Does not react with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Renilla Luciferase transfected HEK293T whole cell lysate. ICC/IF: Renilla Luciferase transfected HEK293T cells. Flow Cyt: Renilla Luciferase transfected HEK293T cells.

  • General notes

    Ab238942 is the carrier-free version of ab185926. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238942 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17792

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)
    Western blot - Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)
    All lanes : Anti-Renilla Luciferase antibody [EPR17792] (ab185926) at 1/1000 dilution (Purified)

    Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), whole cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with Renilla Luciferase expression vector, whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185926).

  • Flow Cytometry - Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)
    Flow Cytometry - Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)

    Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) transfected with GFP tagged Renilla Luciferase cells labeled with purified ab185926 at 1/70 dilution (10µg/ml, Right panel) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185926).

  • Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)
    Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185926 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Renilla Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows;
    1. ab185926 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185936).

  • Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)
    Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free (ab238942)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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