Anti-Renilla Luciferase antibody (ab187338)
Key features and details
- Rabbit polyclonal to Renilla Luciferase
- Suitable for: ICC/IF, WB, IP, Neutralising
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Renilla Luciferase antibody
See all Renilla Luciferase primary antibodies -
Description
Rabbit polyclonal to Renilla Luciferase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IP, Neutralisingmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Renilla Luciferase aa 1-311. (Expressed in E. coli).
Database link: P27652 -
Positive control
- Renilla luciferase transfected 293 whole cell lysate; Recombinant Renilla luciferase; pCMV-Renilla luciferase transfected 293 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Renilla Luciferase antibody (ab187338)All lanes : Anti-Renilla Luciferase antibody (ab187338) at 1/4000 dilution
Lane 1 : Non-transfected 293 whole cell lysate
Lane 2 : Renilla Luciferase transfected 293 whole cell lysate
Lane 3 : Red Firefly Luciferase transfected 293 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
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Western blot - Anti-Renilla Luciferase antibody (ab187338)All lanes : Anti-Renilla Luciferase antibody (ab187338) at 1/4000 dilution
Lane 1 : Non-transfected 293 whole cell lysate
Lane 2 : Renilla Luciferase transfected 293 whole cell lysate
Lane 3 : Not Green-shifted Renilla reniformis Luciferase transfected 293 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
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Western blot - Anti-Renilla Luciferase antibody (ab187338)All lanes : Anti-Renilla Luciferase antibody (ab187338) at 1/4000 dilution
Lane 1 : Full length recombinant Renilla Luciferase at 100 µg
Lane 2 : Full length recombinant Renilla Luciferase at 50 µg
Lane 3 : Full length recombinant Renilla Luciferase at 25 µg
Lane 4 : Full length recombinant Renilla Luciferase at 10 µg
Secondary
All lanes : Goat anti-rabbit HRP at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody (ab187338)Immunofluorescent analysis of Renilla Luciferase (from the Sea pansy Renilla reniformis) in untransfected (left panel) and pCMV-Renilla Luciferase transfected (right panel) 293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were probed with ab187338 at 1/1000 dilution for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody 1/400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
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Immunoprecipitation - Anti-Renilla Luciferase antibody (ab187338)Immunoprecipitation of Renilla Luciferase (from the Sea pansy Renilla reniformis) was performed using whole cell lysates from untransfected and Renilla Luciferase or Red Firefly luciferase transfected 293 cell lines. Antigen-antibody complexes were formed by incubating 200ug of lysate with 5ug of ab187338 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted. Whole cell lysates from untransfected and transfected whole cell lysates (2ug) were used as controls. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with ab187338 at a dilution of 1/4000 overnight rotating at 4°C.
Lane 1: Non-transfected 293 whole cell lysate
Lane 2: Renilla Luciferase IP from non-transfected 293 whole cell lysate
Lane 3: Renilla Luciferase transfected 293 whole cell lysate
Lane 4: Renilla Luciferase IP from Renilla Luciferase transfected 293 whole cell lysate
Lane 5: Red Firefly Luciferase transfected 293 whole cell lysate
Lane 6: Renilla Luciferase IP from Red Firefly Luciferase transfected 293 whole cell lysate
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Neutralising - Anti-Renilla Luciferase antibody (ab187338)Neutralizing effect of Renilla luciferase antibody on the activity of purified recombinant Renilla luciferase (from the Sea pansy Renilla reniformis) using a Renilla Luciferase Assay Kit. 1ng of recombinant enzyme were incubated with the indicated amounts of ab187338 for at least 30 min. Enzyme activity (RLU) was detected. Average RUI values from 10 timepoints per concentration of 4 replicates +/- standard deviation are shown.
