Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17791] to Renilla Luciferase
Suitable for: WB, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-Renilla Luciferase antibody [EPR17791]
See all Renilla Luciferase primary antibodies
Description

Rabbit monoclonal [EPR17791] to Renilla Luciferase
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Renilla Luciferase transfected HEK293T whole cell lysate. ICC/IF: Renilla Luciferase transfected HEK293T cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17791
Isotype

IgG
Research areas

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 15 seconds
Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185925 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK-293T cells transfected with Renilla Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab185925 at 1/1000 dilution followed by ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor^®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) lysate
Lane 5 : Jurkat (Human T cell leukemia cells from peripheral blood) lysate
Lane 6 : SH-SY5Y (Human neuroblastoma from bone marrow cells) lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 20 µg
Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg
Lane 3 : Human fetal brain lysate at 10 µg
Lane 4 : Human fetal heart lysate at 10 µg
Lane 5 : Human fetal kidney lysate at 10 µg
Lane 6 : Human fetal spleen lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse kidney lysate
Lane 6 : Mouse spleen lysate
Lane 7 : Rat brain lysate
Lane 8 : Rat heart lysate
Lane 9 : Rat kidney lysate
Lane 10 : Rat spleen lysate
Lane 11 : C6 (Rat glial tumor cells) lysate
Lane 12 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate
Lane 13 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
Lane 14 : NIH/3T3 (Mouse embyro fibroblast cells) lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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