Anti-Renilla Luciferase antibody [EPR17791] - BSA and Azide free (ab236020)
![Anti-Renilla Luciferase antibody [EPR17791] - BSA and Azide free (ab236020)](https://www.abcam.com/ps/products/236/ab236020/Images/ab236020-311948-23602022.jpg "Anti-Renilla Luciferase antibody [EPR17791] - BSA and Azide free (ab236020)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17791] to Renilla Luciferase - BSA and Azide free
- Suitable for: ICC/IF, WB
Overview
-
Product name
Anti-Renilla Luciferase antibody [EPR17791] - BSA and Azide free
See all Renilla Luciferase primary antibodies -
Description
Rabbit monoclonal [EPR17791] to Renilla Luciferase - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: HEK-293T cells transfected with Renilla Luciferase.
-
General notes
Ab236020 is the carrier-free version of ab185925. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab236020 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17791 -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17791] - BSA and Azide free (ab236020)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185925 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK-293T cells transfected with Renilla Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab185925 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185925).
-
![Anti-Renilla Luciferase antibody [EPR17791] - BSA and Azide free (ab236020)](https://www.abcam.com/ps/products/236/ab236020/Images/ab236020-2-benefits-of-recombinant-antibodies.png)
Anti-Renilla Luciferase antibody [EPR17791] - BSA and Azide free (ab236020)
