Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] - BSA and Azide free (ab247798)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4568] to Muscarinic Acetylcholine Receptor 2/CM2 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] - BSA and Azide free
See all Muscarinic Acetylcholine Receptor 2/CM2 primary antibodies -
Description
Rabbit monoclonal [EPR4568] to Muscarinic Acetylcholine Receptor 2/CM2 - BSA and Azide free -
Host species
Rabbit -
Specificity
Please note: blast analysis of the immunogen sequence for this antibody shows 64% homology with the Muscarinic Acetylcholine Receptor 3, therefore some reactivity with this receptor subtype may be possible.
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Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247798 is the carrier-free version of ab109226. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247798 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR4568 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] - BSA and Azide free (ab247798)All lanes : Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Fetal brain tissue lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 4 : Neuro-2a lysate
Lane 5 : Y79 (Human retinoblastoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaThis data was developed using ab109226, the same antibody clone in a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] - BSA and Azide free (ab247798)This data was developed using ab109226, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109226 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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