Immunocytochemistry staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).

Overlay histogram showing HeLa cells stained with ab27074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27074, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunocytochemisrty analysis of murine fibroblasts labeling gamma Tubulin with ab27074 at 1 ug/ml (red). Nuclei were stained with DAPI (blue).

Immunocytochemisrty analysis of 3T3 mouse fibroblasts in metaphase (A), anaphase (B) and telophase (C), labeling gamma Tubulin with ab27074 at 1 ug/ml (red). Cells were costained for alpha-tubulin (green). Nuclei were stained with DAPI (blue). 

ab27074 staining gamma tubulin in the Human HEK293T cells by ICC. Cells were fixed with paraformaldehyde, permeabilized with methanol (-20ºC 20 min) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 or 1/1000 in blocking solution) for 45 minutes. A Texas Red^®-conjugated Goat anti-mouse polyclonal (1/500) was used as the secondary antibody.

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ab27074 staining gamma tubulin (red) in rat prostate carcinoma cells by ICC. The cells were also transfected with beta-tubulin GFP which stained green.