Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Friday March 05, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3315] to TPPP
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-TPPP antibody [EPR3315]
See all TPPP primary antibodies
Description

Rabbit monoclonal [EPR3315] to TPPP
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain and SH-SY5Y cell lysates; Human astrocytoma tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Previously labelled as Tubulin Polymerization Promoting Protein.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3315
Isotype

IgG
Research areas

Western blot - Anti-TPPP antibody [EPR3315] (ab119987)

Anti-TPPP antibody [EPR3315] (ab119987) at 1/1000 dilution (Purified) + Human brain lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 23 kDa
Observed band size: 25 kDa
why is the actual band size different from the predicted?

Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
Western blot - Anti-TPPP antibody [EPR3315] (ab119987)

All lanes : Anti-TPPP antibody [EPR3315] (ab119987) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 23 kDa

The cell lysates were prepared in 1%SDS Hot lysis method
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3315] (ab119987)

ab119987 at 1/100 dilution staining TPPP in Paraffin-embedded Human astrocytoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-TPPP antibody [EPR3315] (ab119987)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com