Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TPPP antibody [EPR3315] (ab119987)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3315] to TPPP
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-TPPP antibody [EPR3315]
    See all TPPP primary antibodies

  • Description

    Rabbit monoclonal [EPR3315] to TPPP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain and SH-SY5Y cell lysates; Human astrocytoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as Tubulin Polymerization Promoting Protein.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3315

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TPPP antibody [EPR3315] (ab119987)
    Western blot - Anti-TPPP antibody [EPR3315] (ab119987)
    Anti-TPPP antibody [EPR3315] (ab119987) at 1/1000 dilution (Purified) + Human brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 23 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-TPPP antibody [EPR3315] (ab119987)
    Western blot - Anti-TPPP antibody [EPR3315] (ab119987)
    All lanes : Anti-TPPP antibody [EPR3315] (ab119987) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 23 kDa



    The cell lysates were prepared in 1%SDS Hot lysis method

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3315] (ab119987)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3315] (ab119987)

    ab119987 at 1/100 dilution staining TPPP in Paraffin-embedded Human astrocytoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-TPPP antibody [EPR3315] (ab119987)
    Anti-TPPP antibody [EPR3315] (ab119987)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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