Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NXF1 antibody [EPR8009] (ab129160)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8009] to NXF1
  • Suitable for: WB, IP, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-NXF1 antibody [EPR8009]
    See all NXF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8009] to NXF1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NXF1. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, 293T, K-562 and HepG2 lysates. ICC/IF: HeLa cells. Flow Cyt: HepG2 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-NXF1 antibody [EPR8009] (ab129160)
    Western blot - Anti-NXF1 antibody [EPR8009] (ab129160)
    Anti-NXF1 antibody [EPR8009] (ab129160) at 1/2000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NXF1 antibody [EPR8009] (ab129160)
    Immunocytochemistry/ Immunofluorescence - Anti-NXF1 antibody [EPR8009] (ab129160)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NXF1 with purified ab129160 at 1/200 dilution (0.62 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-NXF1 antibody [EPR8009] (ab129160)
    Flow Cytometry - Anti-NXF1 antibody [EPR8009] (ab129160)
    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling NXF1 with purified ab129160 at 1/20 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Western blot - Anti-NXF1 antibody [EPR8009] (ab129160)
    Western blot - Anti-NXF1 antibody [EPR8009] (ab129160)
    All lanes : Anti-NXF1 antibody [EPR8009] (ab129160) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NXF1 antibody [EPR8009] (ab129160)
    Immunocytochemistry/ Immunofluorescence - Anti-NXF1 antibody [EPR8009] (ab129160)
    ab129160 at 1/250 dilution staining NXF1 in HepG2 cells by immunofluorescence.
  • Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (ab129160)
    Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (ab129160)

    ab129160 at 1/20 immunoprecipitating NXF1 in HepG2 (human hepatocellular carcinoma) whole cell lysate observed at 70 KDa (Lanes 1 and 2).

    Lane 1 (input): HepG2 whole cell lysate, 10μg
    Lane 2 (+): ab129160 + HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129160 in HepG2 whole cell lysate

    For western blotting, ab129160 at 1/500 dilution and ab131366 VeriBlot for IP (HRP) at 1/1000 were used.

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (ab129160)
    Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (ab129160)

    ab129160 at 1/20 immunoprecipitating NXF1 in HeLa (human cervix adenocarcinoma) whole cell lysate observed at 70 KDa (Lanes 1 and 2).

    Lane 1 (input): HeLa whole cell lysate, 10μg
    Lane 2 (+): ab129160 + HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129160 in HeLa whole cell lysate

    For western blotting, ab129160 at 1/500 dilution and ab131366 VeriBlot for IP (HRP) at 1/1000 were used.

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

  • Anti-NXF1 antibody [EPR8009] (ab129160)
    Anti-NXF1 antibody [EPR8009] (ab129160)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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