Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-TPPP antibody [EPR3316] - BSA and Azide free
See all TPPP primary antibodies
Description

Rabbit monoclonal [EPR3316] to TPPP - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Human
IHC-P	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human cerebral cortex tissue.
General notes
ab238958 is the carrier-free version of ab92305 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab238958 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Previously labelled as Tubulin Polymerization Promoting Protein.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3316
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-TPPP antibody [EPR3316] - BSA and Azide free (ab238958)

Immunocytochemistry/Immunofluorescence staining of Neuro-2a (mouse neuroblastoma) cells labelling TPPP with purified ab92305 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. DAPI was used as nuclear counterstain. The cells were fixed in 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, rabbit primary antibody was used followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120). For negative control 2, ab7291 (mouse anti-tubulin) was used followed by an Alexa Fluor® 488 goat anti-rabbit secondary (ab150077).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92305).
Flow Cytometry - Anti-TPPP antibody [EPR3316] - BSA and Azide free (ab238958)

Overlay histogram showing 4% paraformaldehyde fixed Neuro-2a (mouse neuroblastoma) cells labelling TPPP with purified ab92305 at dilution of 1/20. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG at dilution of 1/2000. A non-specific IgG antibody (rabbit monoclonal) was used as isotype control (black line). The blue line shows cells without incubation with primary antibody and secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92305).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3316] - BSA and Azide free (ab238958)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue sections labelling TPPP with purified ab92305 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92305).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3316] - BSA and Azide free (ab238958)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue sections labelling TPPP with purified ab92305 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92305).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3316] - BSA and Azide free (ab238958)

Immunohistochemical analysis of paraffin-embedded human glioma tissue sections labelling TPPP with purified ab92305 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92305).
Flow Cytometry - Anti-TPPP antibody [EPR3316] - BSA and Azide free (ab238958)

Overlay histogram showing SH-SY5Y cells stained with ab92305 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92305, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92305).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3316] - BSA and Azide free (ab238958)

ab92305 at 1/250 dilution staining TPPP in paraffin-embedded Human brain tissue by immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92305).

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TPPP antibody [EPR3316] - BSA and Azide free (ab238958)

ab92305 at 1/100 dilution staining TPPP in SH-SY5Y cells, by immunofluorescence.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92305).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3316] - BSA and Azide free (ab238958)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue sections labelling TPPP with purified ab92305 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92305).
Anti-TPPP antibody [EPR3316] - BSA and Azide free (ab238958)