Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase
  • Suitable for: IP, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556]
    See all Tartrate Resistant Acid Phosphatase primary antibodies

  • Description

    Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase

  • Host species

    Rabbit

  • Specificity

    This antibody gave negative results in IHC-P in both mouse and rat species. Both species were also tested in WB with positive results. Please do not hesitate to contact our Scientific Support if you have any questions.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A431, MDA-MB-435, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates; Human tonsil and fetal kidney lysates; Human tonsil tissue; MDA-MB-435 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/10000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : MDA-MB-435 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 35,42 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM /TBST.

     

    Per the literature, a novel previously unreported 42 kDa variant of the TRAP 5a isoform was identified. So the 42 kDa band should be the TRAP 5a isoform.

  • Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized MDA-MB-435 cells labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).

    The two negative controls used anti-TRAP 5 primary antibody at 1/100 dilution, followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.

  • Immunoprecipitation - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Immunoprecipitation - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

    Western blot analysis of TRAP 5 in MDA-MB-435 cell lysate immunoprecipitated using ab191406 at 1/50 dilution (Lane 1). Lane 2: PBS instead of MDA-MB-435 lysates.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution.

  • Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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