Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

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Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase
Suitable for: IP, ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556]
See all Tartrate Resistant Acid Phosphatase primary antibodies
Description

Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase
Host species

Rabbit
Specificity

This antibody gave negative results in IHC-P in both mouse and rat species. Both species were also tested in WB with positive results. Please do not hesitate to contact our Scientific Support if you have any questions.

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- A431, MDA-MB-435, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates; Human tonsil and fetal kidney lysates; Human tonsil tissue; MDA-MB-435 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15556
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

In the negative control PBS was used instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/10000 dilution

Lane 1 : A431 cell lysate
Lane 2 : MDA-MB-435 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa
Observed band size: 35 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM /TBST.
Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution

Lane 1 : Human tonsil lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 37 kDa
Observed band size: 35,42 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM /TBST.

Per the literature, a novel previously unreported 42 kDa variant of the TRAP 5a isoform was identified. So the 42 kDa band should be the TRAP 5a isoform.
Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cell line) cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 37 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized MDA-MB-435 cells labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).

The two negative controls used anti-TRAP 5 primary antibody at 1/100 dilution, followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.
Immunoprecipitation - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

Western blot analysis of TRAP 5 in MDA-MB-435 cell lysate immunoprecipitated using ab191406 at 1/50 dilution (Lane 1). Lane 2: PBS instead of MDA-MB-435 lysates.

Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution.
Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

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