Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] - BSA and Azide free (ab240970)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase - BSA and Azide free
  • Suitable for: IP, WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] - BSA and Azide free
    See all Tartrate Resistant Acid Phosphatase primary antibodies

  • Description

    Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody gave negative results in IHC-P in both mouse and rat species. Both species were also tested in WB with positive results. Please do not hesitate to contact our Scientific Support if you have any questions.

  • Tested applications

    Suitable for: IP, WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue. ICC/IF: MDA-MB-435 cells. IP: Tartrate Resistant Acid Phosphatase IP in MDA-MB-435 cell lysate.

  • General notes

    Ab240970 is the carrier-free version of ab191406. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240970 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] - BSA and Azide free (ab240970)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] - BSA and Azide free (ab240970)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counterstained with hematoxylin.

    In the negative control PBS was used instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191406)

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] - BSA and Azide free (ab240970)
    Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] - BSA and Azide free (ab240970)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-435 cells labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa-Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counterstain DAPI (blue).

    The two negative controls used anti-TRAP 5 primary antibody at 1/100 dilution, followed by Goat anti-mouse IgG (Alexa-Fluor®594) secondary antibody at 1/400 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191406)

  • Immunoprecipitation - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] - BSA and Azide free (ab240970)
    Immunoprecipitation - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] - BSA and Azide free (ab240970)

    Western blot analysis of TRAP 5 in MDA-MB-435 cell lysate immunoprecipitated using ab191406 at 1/50 dilution (Lane 1). Lane 2: PBS instead of MDA-MB-435 lysates.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191406)

  • Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] - BSA and Azide free (ab240970)
    Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] - BSA and Azide free (ab240970)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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