Anti-EGF antibody [EPR21926] - BSA and Azide free (ab233699)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21926] to EGF - BSA and Azide free
- Suitable for: WB, IHC-Fr, IP, IHC-P
- Reacts with: Rat
Overview
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Product name
Anti-EGF antibody [EPR21926] - BSA and Azide free
See all EGF primary antibodies -
Description
Rabbit monoclonal [EPR21926] to EGF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, IP, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Rat kidney tissue.
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General notes
ab233699 is the carrier-free version of ab218831.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21926 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR21926] - BSA and Azide free (ab233699)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling EGF with ab218831 at 1/10,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in distal convoluted tubules of rat kidney (PMID 1930151) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218831).
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EGF was immunoprecipitated from 0.35 mg of rat salivary gland lysate with ab218831 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218831 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: Rat salivary gland tissue 10 µg (Input).
Lane 2: ab218831 IP in rat salivary gland lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218831 in rat salivary gland lysate.
Blocking/ Dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218831).
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Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR21926] - BSA and Azide free (ab233699)
Immunohistochemical analysis of frozen rat kidney tissue labeling EGF with ab218831 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on distal convoluted tubule of rat kidney (PMID: 23143303) is observed. Tissue was fixed in 4% PFA and permeabilized using 0.2% Triton X-100.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
Perform heat-mediated antigen retrieval by sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218831).
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