Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (ab250864)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14166] to HSPA4 - BSA and Azide free
- Suitable for: IHC-P, ICC, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-HSPA4 antibody [EPR14166] - BSA and Azide free
See all HSPA4 primary antibodies -
Description
Rabbit monoclonal [EPR14166] to HSPA4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250864 is the carrier-free version of ab185962. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250864 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14166 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-HSPA4 antibody [EPR14166] (ab185962) at 1/10000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 94 kDaThis data was developed using ab185962, the same antibody clone in a different buffer formulation.
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This data was developed using ab185962, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of -20°C acetone-fixed HeLa cells labeling HSPA4 with ab185962 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).
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All lanes : Anti-HSPA4 antibody [EPR14166] (ab185962) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 94 kDaThis data was developed using ab185962, the same antibody clone in a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (ab250864)This data was developed using ab185962, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling HSPA4 with ab185962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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This data was developed using ab185962, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 cells labeling HSPA4 with ab185962 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).
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This data was developed using ab185962, the same antibody clone in a different buffer formulation.Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling HSPA4 with ab185962 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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