Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CHD3 antibody [EPNCIR110A] (ab109195)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR110A] to CHD3
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-CHD3 antibody [EPNCIR110A]
    See all CHD3 primary antibodies

  • Description

    Rabbit monoclonal [EPNCIR110A] to CHD3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, HeLa, C6, and PC12 cell lysates, Human breast and Human ovary tissues; HeLa cells

  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
    Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CHD3 knockout HAP1 cell lysate (20 µg)
    Lane 3: Raji cell lysate (20 µg)
    Lane 4: Y79 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)

    Immunohistochemical staining of paraffin-embedded Human breast tissue using ab109195 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
    Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] (ab109195)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CHD3 with purified ab109195 at 1/230 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
    Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
    All lanes : Anti-CHD3 antibody [EPNCIR110A] (ab109195) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : PC12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 227 kDa
    Observed band size: 260 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)

    Immunohistochemical staining of paraffin-embedded Human ovary tissue using ab109195 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
    Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
    Immunofluorescent staining of HeLa cells using ab109195 at a dilution of 1/100.
  • Anti-CHD3 antibody [EPNCIR110A] (ab109195)
    Anti-CHD3 antibody [EPNCIR110A] (ab109195)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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