Anti-RGS16 antibody [OTI4E5] (ab119424)
![Anti-RGS16 antibody [OTI4E5] (ab119424)](https://www.abcam.com/ps/products/119/ab119424/Images/ab119424-132739-anti-rgs16-antibody-oti4e5-western-blot.jpg "Anti-RGS16 antibody [OTI4E5] (ab119424)")
Key features and details
- Mouse monoclonal [OTI4E5] to RGS16
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Dog, Human, African green monkey
- Isotype: IgG1
Overview
-
Product name
Anti-RGS16 antibody [OTI4E5] -
Description
Mouse monoclonal [OTI4E5] to RGS16 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF African green monkeyIHC-P HumanWB Human
-
Immunogen
Recombinant full length protein corresponding to Human RGS16 aa 1-250. Produced in HEK293T cells (NP_002919).
Database link: O15492 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY RGS16 cDNA; SVT2 cell extract. IHC-P: Human breast, Kidney, Pancreas and Endometrium tissues. ICC/IF: COS7 cells transiently transfected with pCMV6-ENTRY RGS16. Flow: Jurkat cells.
-
General notes
clone OTI4E5 (formerly 4E5)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI4E5 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-RGS16 antibody [OTI4E5] (ab119424)All lanes : Anti-RGS16 antibody [OTI4E5] (ab119424) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY RGS16 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 23 kDa
-
![Western blot - Anti-RGS16 antibody [OTI4E5] (ab119424)](https://www.abcam.com/ps/products/119/ab119424/Images/ab119424-132738-anti-rgs16-antibody-oti4e5-western-blot.jpg)
Western blot - Anti-RGS16 antibody [OTI4E5] (ab119424)All lanes : Anti-RGS16 antibody [OTI4E5] (ab119424) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 7 : MDCK cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 23 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)](https://www.abcam.com/ps/products/119/ab119424/Images/ab119424-132737-anti-rgs16-antibody-oti4e5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)Paraffin-embedded human breast tissue stained for RGS16 using ab119424 at 1/150 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)](https://www.abcam.com/ps/products/119/ab119424/Images/ab119424-132736-anti-rgs16-antibody-oti4e5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)Paraffin-embedded human kidney tissue stained for RGS16 using ab119424 at 1/150 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)](https://www.abcam.com/ps/products/119/ab119424/Images/ab119424-132735-anti-rgs16-antibody-oti4e5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)Paraffin-embedded human pancreas tissue stained for RGS16 using ab119424 at 1/150 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)](https://www.abcam.com/ps/products/119/ab119424/Images/ab119424-132734-anti-rgs16-antibody-oti4e5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)Paraffin-embedded human endometrium tissue stained for RGS16 using ab119424 at 1/150 dilution in immunohistochemical analysis.
-
![Immunocytochemistry/ Immunofluorescence - Anti-RGS16 antibody [OTI4E5] (ab119424)](https://www.abcam.com/ps/products/119/ab119424/Images/ab119424-132733-anti-rgs16-antibody-oti4e5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RGS16 antibody [OTI4E5] (ab119424)COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY RGS16, stained for RGS16 (green) using ab119424 at 1/100 dilution in ICC/IF.
-
![Flow Cytometry - Anti-RGS16 antibody [OTI4E5] (ab119424)](https://www.abcam.com/ps/products/119/ab119424/Images/ab119424-132732-anti-rgs16-antibody-oti4e5-flow-cytometry.jpg)
Flow Cytometry - Anti-RGS16 antibody [OTI4E5] (ab119424)Flow cytometric analysis of jurkat (human T cell leukemia cell line from peripheral blood) cells staining RGS16 (red) using ab119424 at 1/100 dilution compared to a nonspecific negative control antibody (Blue).
