Anti-HtrA2 / Omi antibody - C-terminal (ab229878)
Key features and details
- Rabbit polyclonal to HtrA2 / Omi - C-terminal
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HtrA2 / Omi antibody - C-terminal
See all HtrA2 / Omi primary antibodies -
Description
Rabbit polyclonal to HtrA2 / Omi - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Recombinant fragment corresponding to Human HtrA2/ Omi aa 300 to the C-terminus (C terminal).
Database link: O43464 -
Positive control
- WB: HeLa, HEK-293, Jurkat, MCF7 and Raji whole cell lysates. IHC-P: Human colon cancer and kidney tissues. IP: MCF7 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HtrA2 / Omi antibody - C-terminal (ab229878)All lanes : Anti-HtrA2 / Omi antibody - C-terminal (ab229878) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody - C-terminal (ab229878)Paraffin-embedded human colon cancer tissue stained for HtrA2 / Omi using ab229878 at 1/200 dilution in immunohistochemical analysis, followed by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight.
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Immunoprecipitation - Anti-HtrA2 / Omi antibody - C-terminal (ab229878)HtrA2 / Omi was immunoprecipitated from 0.5 mg MCF7 (human breast adenocarcinoma cell line) whole cell lysate using 8 µg of ab229878. Western blot was performed from the immunoprecipitate using ab229878. HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
Lane 1: 1 µg Control IgG instead of ab229878 in MCF7 whole cell lysate.
Lane 2: ab229878 in MCF7 whole cell lysate.
Lane 3: MCF7 whole cell lysate 20 µg (Input). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody - C-terminal (ab229878)Paraffin-embedded human kidney tissue stained for HtrA2 / Omi using ab229878 at 1/200 dilution in immunohistochemical analysis, followedby a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight.
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Western blot - Anti-HtrA2 / Omi antibody - C-terminal (ab229878)All lanes : Anti-HtrA2 / Omi antibody - C-terminal (ab229878) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
