Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR110A] to CHD3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free
    See all CHD3 primary antibodies

  • Description

    Rabbit monoclonal [EPNCIR110A] to CHD3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild Type HAP1 cell lysates. Raji and Y79 cell lysate. IHC-P: Human breast and ovary tissues. Flow Cyt (intra): HeLa cells.

  • General notes

    ab240942 is the carrier-free version of ab109195 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240942 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
    Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
    Immunofluorescent staining of HeLa cells using ab109195 at a dilution of 1/100.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)
  • Western blot - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
    Western blot - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CHD3 knockout HAP1 cell lysate (20 µg)
    Lane 3: Raji cell lysate (20 µg)
    Lane 4: Y79 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab109195).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

    Immunohistochemical staining of paraffin-embedded Human ovary tissue using ab109195 at a dilution of 1/100.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

    Immunohistochemical staining of paraffin-embedded Human breast tissue using ab109195 at a dilution of 1/100.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
    Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CHD3 with purified ab109195 at 1/230 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)
  • Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
    Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

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