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Immunology Innate Immunity Macrophage / Inflamm.

Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)

Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [C1] to Cytosolic Phospholipase A2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-Cytosolic Phospholipase A2 antibody [C1]
    See all Cytosolic Phospholipase A2 primary antibodies
  • Description

    Mouse monoclonal [C1] to Cytosolic Phospholipase A2
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human Cytosolic Phospholipase A2 aa 550-750. N-Terminal His-tag. Expressed in E.coli
    Database link: P47712

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Recombinant human Cytosolic Phospholipase A2 protein; human lung lysate; PC3 and HeLa cell lysates. IHC-P: Human kidney, pancreas, glioma and stomach tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    C1
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLA
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)

    Paraffin-embedded human glioma tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)
    Western blot - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)
    All lanes : Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730) at 1 µg/ml

    Lane 1 : Human lung lysate
    Lane 2 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Secondary
    All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

    Predicted band size: 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)

    Paraffin-embedded human kidney tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)
    Western blot - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)
    Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730) at 5 µg/ml + Recombinant human Cytosolic Phospholipase A2 protein

    Predicted band size: 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)

    Paraffin-embedded human pancreas tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)

    Paraffin-embedded human stomach tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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