Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)
Key features and details
- Mouse monoclonal [C1] to Cytosolic Phospholipase A2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Cytosolic Phospholipase A2 antibody [C1]
See all Cytosolic Phospholipase A2 primary antibodies -
Description
Mouse monoclonal [C1] to Cytosolic Phospholipase A2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human Cytosolic Phospholipase A2 aa 550-750. N-Terminal His-tag. Expressed in E.coli
Database link: P47712 -
Positive control
- WB: Recombinant human Cytosolic Phospholipase A2 protein; human lung lysate; PC3 and HeLa cell lysates. IHC-P: Human kidney, pancreas, glioma and stomach tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Paraffin-embedded human glioma tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730) at 1 µg/ml
Lane 1 : Human lung lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 85 kDa
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Paraffin-embedded human kidney tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730) at 5 µg/ml + Recombinant human Cytosolic Phospholipase A2 protein
Predicted band size: 85 kDa
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Paraffin-embedded human pancreas tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human stomach tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.