Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105)
Key features and details
- Rabbit polyclonal to Cytosolic Phospholipase A2 (phospho S505)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Cytosolic Phospholipase A2 (phospho S505) antibody
See all Cytosolic Phospholipase A2 primary antibodies -
Description
Rabbit polyclonal to Cytosolic Phospholipase A2 (phospho S505) -
Host species
Rabbit -
Specificity
ab53105 detects endogenous levels of Cytosolic Phospholipase A2 only when phosphorylated at serine 505. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Cytosolic Phospholipase A2 (phospho S505).
Sequence:P-L-Sp-P-L
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53105 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105)All lanes : Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105) at 1/300 dilution
Lane 1 : extracts from HeLa cells treated
with TNF-a (20ng/ml, 30mins)
Lane 2 : extracts from HeLa cells treated
with TNF-a (20ng/ml, 30mins) with immunising peptide
Predicted band size: 85 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105)ab53105 at 1/50 dilution staining Cytosolic Phospholipase A2 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
-
Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105)ICC/IF image of ab53105 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53105, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
