Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CCR2 antibody [EPR19698] (ab203128)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19698] to CCR2
Suitable for: ICC/IF, WB
Reacts with: Mouse

Product name

Anti-CCR2 antibody [EPR19698]
See all CCR2 primary antibodies
Description

Rabbit monoclonal [EPR19698] to CCR2
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Mouse

WB
Mouse

See all applications and species data
Immunogen

This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

Recombinant fragment within Mouse CCR2 aa 300 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P51683

Run BLAST with Run BLAST with
Positive control
- WB: Mouse spleen and brain lysates; RAW 264.7 and NIH/3T3 whole cell lysates; J774A.1 whole cell lysates untreated and treated with 500U/ml IFN gamma for 4 hours. ICC/IF: RAW 264.7 and J774A.1 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19698
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on J774A.1 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)

All lanes : Anti-CCR2 antibody [EPR19698] (ab203128) at 1/1000 dilution

Lane 1 : Mouse spleen lysate
Lane 2 : Mouse brain lysate
Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] (ab203128)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on RAW 264.7 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Western blot - Anti-CCR2 antibody [EPR19698] (ab203128)

All lanes : Anti-CCR2 antibody [EPR19698] (ab203128) at 1/5000 dilution

Lane 1 : Untreated J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
Lane 2 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) treated with 500U/ml IFN gamma for 4 hours whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Pro-inflammatory cytokines such as IFN-gamma, IL-1, or TNF treatment decreases CCR2 expression (PMID: 9558092).
Anti-CCR2 antibody [EPR19698] (ab203128)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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