Anti-CCR2 antibody [EPR19698] - BSA and Azide free (ab271969)
![Anti-CCR2 antibody [EPR19698] - BSA and Azide free (ab271969)](https://www.abcam.com/ps/products/271/ab271969/Images/ab271969-2-ab203128266591anticcr2antibodyepr19698immunofluorescence.jpg "Anti-CCR2 antibody [EPR19698] - BSA and Azide free (ab271969)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19698] to CCR2 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
Overview
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Product name
Anti-CCR2 antibody [EPR19698] - BSA and Azide free
See all CCR2 primary antibodies -
Description
Rabbit monoclonal [EPR19698] to CCR2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Mouse
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Immunogen
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment within Mouse CCR2 aa 300 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P51683 -
Positive control
- ICC/IF: RAW 264.7 and J774A.1 cells.
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General notes
ab271969 is the carrier-free version of ab203128. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19698 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] - BSA and Azide free (ab271969)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on RAW 264.7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203128). -
![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] - BSA and Azide free (ab271969)](https://www.abcam.com/ps/products/271/ab271969/Images/ab271969-1-ab203128266590anticcr2antibodyepr19698immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] - BSA and Azide free (ab271969)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on J774A.1 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203128). -
![Anti-CCR2 antibody [EPR19698] - BSA and Azide free (ab271969)](https://www.abcam.com/ps/products/271/ab271969/Images/ab271969-3-benefits-of-recombinant-antibodies.png)
Anti-CCR2 antibody [EPR19698] - BSA and Azide free (ab271969)
