Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23677-111] to S100A12/CGRP
  • Suitable for: WB, IHC-P, Flow Cyt, IP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-S100A12/CGRP antibody [EPR23677-111]
    See all S100A12/CGRP primary antibodies

  • Description

    Rabbit monoclonal [EPR23677-111] to S100A12/CGRP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human spleen and lymph node tissue lysate. IHC-P: Human spleen tissue. Human bladder cancer and cervical cancer tissue. ICC/IF: Human PBMCs. Flow Cyt: Human PBMCs. IP: Human lymph node tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
    Western blot - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
    All lanes : Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713) at 1/1000 dilution

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human lymph node tissue lysate
    Lane 3 : Human brain tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19542470).

    Negative control: Human brain (PMID: 8718672).

    Exposure time: 103 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human spleen (PMID: 10464253). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
    Immunocytochemistry/ Immunofluorescence - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized human PBMC cells labelling S100A12/CGRP with ab272713 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in subsets of human PBMC cell line. ab195889 anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

  • Flow Cytometry - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
    Flow Cytometry - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) cells labeling S100A12/CGRP with ab272713 at 1/400 dilution (0.1µg) (Right panel), compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left panel). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
    Immunoprecipitation - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

    S100A12/CGRP was immunoprecipitated from 0.35 mg human lymph node tissue lysate 10 µg with ab272713 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272713 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human lymph node tissue lysate 10 µg.

    Lane 2: ab272713 IP in Human lymph node tissue lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272713 in human lymph node tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 50 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human bladder cancer (PMID: 31993369). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human cervical cancer (PMID: 31993369). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
    Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

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