Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

Order now and get it on Tuesday March 09, 2021

Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23677-111] to S100A12/CGRP
Suitable for: WB, IHC-P, Flow Cyt, IP, ICC/IF
Reacts with: Human

Product name

Anti-S100A12/CGRP antibody [EPR23677-111]
See all S100A12/CGRP primary antibodies
Description

Rabbit monoclonal [EPR23677-111] to S100A12/CGRP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human spleen and lymph node tissue lysate. IHC-P: Human spleen tissue. Human bladder cancer and cervical cancer tissue. ICC/IF: Human PBMCs. Flow Cyt: Human PBMCs. IP: Human lymph node tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23677-111
Isotype

IgG
Research areas

Western blot - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

All lanes : Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713) at 1/1000 dilution

Lane 1 : Human spleen tissue lysate
Lane 2 : Human lymph node tissue lysate
Lane 3 : Human brain tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 10 kDa
Observed band size: 10 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 19542470).

Negative control: Human brain (PMID: 8718672).

Exposure time: 103 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human spleen (PMID: 10464253). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized human PBMC cells labelling S100A12/CGRP with ab272713 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in subsets of human PBMC cell line. ab195889 anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 2 µg/ml dilution.
Flow Cytometry - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) cells labeling S100A12/CGRP with ab272713 at 1/400 dilution (0.1µg) (Right panel), compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left panel). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

S100A12/CGRP was immunoprecipitated from 0.35 mg human lymph node tissue lysate 10 µg with ab272713 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272713 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human lymph node tissue lysate 10 µg.

Lane 2: ab272713 IP in Human lymph node tissue lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272713 in human lymph node tissue lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 50 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human bladder cancer (PMID: 31993369). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human cervical cancer (PMID: 31993369). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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