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Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)

Key features and details

  • Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)
  • Suitable for: IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387]
    See all S100A9 + Calprotectin (S100A8/A9 complex) primary antibodies

  • Description

    Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Human S100A9 + Calprotectin (S100A8/A9 complex). Affinity purified solubilized human monocyte preparation.

  • Positive control

    • IHC-P: Mouse spleen tissue; human Hodgkin's lymphoma tissue.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    MAC387

  • Myeloma

    NS1

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506) This image is courtesy of an Abreview submitted by Elizabeth Chlipala

    ab22506 staining Macrophages in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.250% casein for 5 minutes at 25°C; antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/5000 in Tris-HCL) for 30 minutes at 25°C. A HRP-conjugated labeled polymer was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)
    IHC image of Macrophage staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)
    IHC image of Macrophage staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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