Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3555] to S100A9 - Low endotoxin, Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free
    See all S100A9 primary antibodies

  • Description

    Rabbit monoclonal [EPR3555] to S100A9 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Breast cancer, Human tonsil, Human spleen and peripheral blood lymphocytes lysates, Human breast carcinoma tissue and Human spleen tissue.IF: DU145 cell lineFlow Cyt: Jurkat cells

  • General notes

    ab227570 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)

    Anti-S100A9 antibody [EPR3555] (ab92507)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling S100A9 with ab92507 at a dilution of 1:500. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
    This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)

    This IHC data was generated using the same anti-S100A9 antibody clone, EPR3555, in a different buffer formulation (cat# ab92507).

    ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

  • Flow Cytometry - Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)
    Flow Cytometry - Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)

    Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling S100A9 (red) with purified ab92507 at a 1/200 dilution (10ug/mL). Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92507).

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)
    Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)
    ICC/IF image of ab92507 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92507, 1/200) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgM (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92507).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)

    This IHC data was generated using the same anti-S100A9 antibody clone, EPR3555, in a different buffer formulation (cat# ab92507).

    ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human spleen tissue by Immunohistochemistry.

  • Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)
    Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)

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