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Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22332-75] to S100A9 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free
    See all S100A9 primary antibodies

  • Description

    Rabbit monoclonal [EPR22332-75] to S100A9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
    Unsuitable for: IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse spleen tissue; Rat spleen tissue. ICC/IF: Mouse spleen cells. Flow cyt: Mouse primary splenocytes.

  • General notes

    Ab243108 is the carrier-free version of ab242945. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab243108 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling S100A9 with ab242945 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on granulocytes of rat spleen (PMID: 25860480) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242945).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling S100A9 with ab242945 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on granulocytes of mouse spleen (PMID: 25860480) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242945).
  • Flow Cytometry - Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)
    Flow Cytometry - Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse primary splenocytes labeling S100A9 with ab242945 at 1/600 dilution (right panel) compared with an Isotype control Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242945).

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)
    Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse spleen cells labeling S100A9 with ab242945 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of spleen cells (PMID: 2666142) is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242945).

  • Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)
    Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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