Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3158Y] to PU.1/Spi1 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IHC-Fr
  • Reacts with: Human

Overview

  • Product name

    Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free
    See all PU.1/Spi1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3158Y] to PU.1/Spi1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: U-937 cells

  • General notes

    ab232383 is the carrier-free version of ab76543 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232383 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)
    ab76543 showing positive staining in Normal spleen tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76543).

  • Flow Cytometry - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)
    Flow Cytometry - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)

    This data was developed using ab76543, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling PU.1/Spi1 with purified ab76543 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)
    ab76543 showing positive staining in Normal lung lymphocytes staining tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76543).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)
    ab76543 showing negative staining in Normal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76543).

  • OI-RD Scanning - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)
    OI-RD Scanning - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76543).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for PU.1/Spi1 with ab76543 (1/100 dilution) in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76543).

  • Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)
    Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (ab232383)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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