Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3158Y] to PU.1/Spi1
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-PU.1/Spi1 antibody [EPR3158Y]
See all PU.1/Spi1 primary antibodies -
Description
Rabbit monoclonal [EPR3158Y] to PU.1/Spi1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human PU.1/Spi1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P17947 -
Positive control
- Daudi and U937 cell lysates. Human tonsil tissue. Flow Cyt: U-937 cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3158Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) at 1/10000 dilution
Lane 1 : Daudi cell lysates
Lane 2 : U937 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
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ab76543 showing positive staining in Normal spleen tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling PU.1/Spi1 with purified ab76543 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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ab76543, at 1/100 dilution, staining PU.1/Spi1 in paraffin-embedded human tonsil tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab76543 showing positive staining in Normal lung lymphocytes staining tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab76543 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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