Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11233(2)] to ITIH4
Suitable for: IP, IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-ITIH4 antibody [EPR11233(2)]
See all ITIH4 primary antibodies
Description

Rabbit monoclonal [EPR11233(2)] to ITIH4
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment within Human ITIH4 aa 1-150. The exact sequence is proprietary.
Database link: Q14624
Positive control
- Human and mouse plasma, Mouse and rat serum, Human liver and placenta tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11233(2)
Isotype

IgG
Research areas

Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

All lanes : Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution

Lane 1 : Mouse serum
Lane 2 : Mouse plasma
Lane 3 : Rat serum

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000 µg

Predicted band size: 103 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?

Blocking buffer and concentration: 5%NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

Western blot analysis of ITIH4 in immunoprecipitation pellets from Human plasma lysate. ab180139 used at 1/60 dilution for immunoprecipitation. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution + Human plasma at 10 µg

Secondary
Anti-Rabbit IgG (HRP) at 1/1000 dilution

Predicted band size: 103 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5%NFDM /TBST
Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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