Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11233(2)] to ITIH4
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ITIH4 antibody [EPR11233(2)]
    See all ITIH4 primary antibodies

  • Description

    Rabbit monoclonal [EPR11233(2)] to ITIH4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human ITIH4 aa 1-150. The exact sequence is proprietary.
    Database link: Q14624

  • Positive control

    • Human and mouse plasma, Mouse and rat serum, Human liver and placenta tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11233(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    All lanes : Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution

    Lane 1 : Mouse serum
    Lane 2 : Mouse plasma
    Lane 3 : Rat serum

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000 µg

    Predicted band size: 103 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5%NFDM /TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

    Western blot analysis of ITIH4 in immunoprecipitation pellets from Human plasma lysate. ab180139 used at 1/60 dilution for immunoprecipitation. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution + Human plasma at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP) at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5%NFDM /TBST

  • Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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