Anti-TLR2 antibody [TL2.1] (ab9100)
![Anti-TLR2 antibody [TL2.1] (ab9100)](https://www.abcam.com/ps/products/9/ab9100/Images/ab9100-16776-anti-tlr2-antibody-tl21-flow-cytometry.jpg "Anti-TLR2 antibody [TL2.1] (ab9100)")
Key features and details
- Mouse monoclonal [TL2.1] to TLR2
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-TLR2 antibody [TL2.1]
See all TLR2 primary antibodies -
Description
Mouse monoclonal [TL2.1] to TLR2 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.
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Positive control
- WB: THP1 + LPS. IHC-P: Human TLR2. ICC: Human U937 cells. Flow: Human peripheral blood mononuclear cells.
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General notes
Ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2 µm filtered -
Primary antibody notes
Ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells. -
Clonality
Monoclonal -
Clone number
TL2.1 -
Isotype
IgG2a -
Research areas
Images
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Flow Cytometry - Anti-TLR2 antibody [TL2.1] (ab9100)TLR2 staining on human peripheral blood mononuclear cells by using ab9100. Green: CD3+-cells; Red: CD14+ cells
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![Western blot - Anti-TLR2 antibody [TL2.1] (ab9100)](https://www.abcam.com/ps/products/9/ab9100/Images/ab9100-256365-anti-tlr2-antibody-tl21-western-blot.jpg)
Western blot - Anti-TLR2 antibody [TL2.1] (ab9100)All lanes : Anti-TLR2 antibody [TL2.1] (ab9100) at 1 µg/ml
Lane 1 : THP1
Lane 2 : THP1 + LPS
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Performed under reducing conditions.This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was ran at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked with 2% BSA for 60 minutes before being incubated with ab9100 (anti-TLR2 antibody; 1 ug/mL) for 18 hours at 4°C. Antibody binding was detected using ab97040 (HRP-labelled goat anti-mouse IgG) at 1:50,000 dilutions for 1 hour at room temperature.Visualisation was conducted using ECL development solution ab133406.
This gel was run under reducing conditions resulting in weak signal. We recommend customers to use non-reducing conditions.
