Anti-TLR2 antibody [EPR20302-119] - BSA and Azide free (ab243291)
![Anti-TLR2 antibody [EPR20302-119] - BSA and Azide free (ab243291)](https://www.abcam.com/ps/products/243/ab243291/Images/ab243291-332665-anti-tlr2-antibody-epr20302-119-immunofluorescence.jpg "Anti-TLR2 antibody [EPR20302-119] - BSA and Azide free (ab243291)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20302-119] to TLR2 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse
Overview
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Product name
Anti-TLR2 antibody [EPR20302-119] - BSA and Azide free
See all TLR2 primary antibodies -
Description
Rabbit monoclonal [EPR20302-119] to TLR2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse liver and lung tissues. ICC/IF: RAW 264.7 cels.
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General notes
Ab243291 is the carrier-free version of ab209216. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab243291 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20302-119 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TLR2 antibody [EPR20302-119] - BSA and Azide free (ab243291)
Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling TLR2 with ab209216 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in RAW 264.7 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody followed by anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Fixation with 4% PFA is not recommended as no staining was obtained.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209216).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] - BSA and Azide free (ab243291)](https://www.abcam.com/ps/products/243/ab243291/Images/ab243291-332664-anti-tlr2-antibody-epr20302-119-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] - BSA and Azide free (ab243291)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling TLR2 with ab209216 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on macrophages of mouse lung (PMID: 24371556). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab209216 for 10 minutes at 37℃.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209216).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] - BSA and Azide free (ab243291)](https://www.abcam.com/ps/products/243/ab243291/Images/ab243291-332663-anti-tlr2-antibody-epr20302-119-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] - BSA and Azide free (ab243291)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TLR2 with ab209216 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on Kuffer cells of mouse liver (PMID: 21966468). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab209216 for 10 minutes at 37℃.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209216).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-TLR2 antibody [EPR20302-119] - BSA and Azide free (ab243291)](https://www.abcam.com/ps/products/243/ab243291/Images/ab243291-5-benefits-of-recombinant-antibodies.png)
Anti-TLR2 antibody [EPR20302-119] - BSA and Azide free (ab243291)
