Anti-TLR2 antibody [EPR20303] - BSA and Azide free (ab271986)
![Anti-TLR2 antibody [EPR20303] - BSA and Azide free (ab271986)](https://www.abcam.com/ps/products/271/ab271986/Images/ab271986-1-ab209217272119antitlr2antibodyepr20303immunoprecipitation.jpg "Anti-TLR2 antibody [EPR20303] - BSA and Azide free (ab271986)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20303] to TLR2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Overview
-
Product name
Anti-TLR2 antibody [EPR20303] - BSA and Azide free
See all TLR2 primary antibodies -
Description
Rabbit monoclonal [EPR20303] to TLR2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Mouse
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse and rat spleen lysates; RAW 264.7 whole cell lysates untreated and treated with 1 µg/ml lipopolysaccharides for 6 hours IP: RAW 264.7 treated with 1 µg/ml LPS for 6 hours whole cell lysate.
-
General notes
ab271986 is the carrier-free version of ab209217. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20303 -
Isotype
IgG -
Research areas
Images
-
Immunoprecipitation - Anti-TLR2 antibody [EPR20303] - BSA and Azide free (ab271986)
TLR2 was immunoprecipitated from 0.35 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate, treated with 1 µg/ml LPS for 6 hours, with ab209217 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab209217 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours, 10 µg (Input).
Lane 2: ab209217 IP in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209217 in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209217). -
![Anti-TLR2 antibody [EPR20303] - BSA and Azide free (ab271986)](https://www.abcam.com/ps/products/271/ab271986/Images/ab271986-2-benefits-of-recombinant-antibodies.png)
Anti-TLR2 antibody [EPR20303] - BSA and Azide free (ab271986)
