Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100A9 antibody [EPR22332-75] (ab242945)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22332-75] to S100A9
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-S100A9 antibody [EPR22332-75]
    See all S100A9 primary antibodies

  • Description

    Rabbit monoclonal [EPR22332-75] to S100A9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse spleen and lung lysate; Rat spleen lysate. IHC-P: Mouse spleen tissue; Rat spleen tissue. ICC/IF: Mouse spleen cells. Flow cyt: Mouse primary splenocytes.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-S100A9 antibody [EPR22332-75] (ab242945)
    Western blot - Anti-S100A9 antibody [EPR22332-75] (ab242945)
    All lanes : Anti-S100A9 antibody [EPR22332-75] (ab242945) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa



    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

    Exposure times:

    Lane 1:3 minutes;

    Lane 2:169 seconds;

    Lane 3 was developed using a higher sensitivity ECL substrate.

    Lane 3:125 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR22332-75] (ab242945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR22332-75] (ab242945)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling S100A9 with ab242945 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on granulocytes of rat spleen (PMID: 25860480) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR22332-75] (ab242945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR22332-75] (ab242945)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling S100A9 with ab242945 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on granulocytes of mouse spleen (PMID: 25860480) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Flow Cytometry - Anti-S100A9 antibody [EPR22332-75] (ab242945)
    Flow Cytometry - Anti-S100A9 antibody [EPR22332-75] (ab242945)

    Flow cytometric analysis of 2% paraformaldehyde-fixed (for 10 minutes), 0.1% Tween-20 permeabilized mouse primary splenocytes labeling S100A9 with ab242945 at 1/600 dilution (right panel) compared with an Isotype control Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [EPR22332-75] (ab242945)
    Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [EPR22332-75] (ab242945)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse spleen cells labeling S100A9 with ab242945 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of spleen cells (PMID: 2666142) is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

     

  • Anti-S100A9 antibody [EPR22332-75] (ab242945)
    Anti-S100A9 antibody [EPR22332-75] (ab242945)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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