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Immunology Innate Immunity Macrophage / Inflamm.

Anti-CD204 antibody [004] - BSA and Azide free (ab277135)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-CD204 antibody [004] - BSA and Azide free (ab277135)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [004] to CD204 - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-CD204 antibody [004] - BSA and Azide free
    See all CD204 primary antibodies
  • Description

    Rabbit monoclonal [004] to CD204 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Mouse CD204 aa 83-354.
    Database link: P30204

  • Positive control

    • Flow cyt: Raw264.7 cells. ICC: Raw264.7 cells. IHC-P: Mouse liver tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituent: 100% PBS

    0.2 µm filtered solution
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    004
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Innate and adaptive immunity
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Images

  • Flow Cytometry - Anti-CD204 antibody (ab277135)
    Flow Cytometry - Anti-CD204 antibody (ab277135)

    Flow cytometric analysis of CD204 expression on Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells. Cells were stained with ab277135 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also staibed with an isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab277135)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab277135)

    Formalin-fixed, paraffin-embedded embedded mouse liver tissue stained for CD204 using ab277135 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry - Anti-CD204 antibody (ab277135)
    Immunocytochemistry - Anti-CD204 antibody (ab277135)

    Confocal immunofluorescence analysis of CD204 in RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ab277135 at 1/100 dilution at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). 

  • Anti-CD204 antibody [004] - BSA and Azide free (ab277135)
    Anti-CD204 antibody [004] - BSA and Azide free (ab277135)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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