Anti-CD204 antibody [004] - BSA and Azide free (ab277135)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [004] to CD204 - BSA and Azide free
- Suitable for: IHC-P, ICC, Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-CD204 antibody [004] - BSA and Azide free
See all CD204 primary antibodies -
Description
Rabbit monoclonal [004] to CD204 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse CD204 aa 83-354.
Database link: P30204 -
Positive control
- Flow cyt: Raw264.7 cells. ICC: Raw264.7 cells. IHC-P: Mouse liver tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS
0.2 µm filtered solution -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
004 -
Isotype
IgG -
Research areas
Images
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Flow cytometric analysis of CD204 expression on Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells. Cells were stained with ab277135 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also staibed with an isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Formalin-fixed, paraffin-embedded embedded mouse liver tissue stained for CD204 using ab277135 at 1/100 dilution in immunohistochemical analysis.
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Confocal immunofluorescence analysis of CD204 in RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ab277135 at 1/100 dilution at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
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