Anti-Chondroitinase ABC I antibody [HL2010] (ab256485)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [HL2010] to Chondroitinase ABC I
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
Overview
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Product name
Anti-Chondroitinase ABC I antibody [HL2010]
See all Chondroitinase ABC I primary antibodies -
Description
Mouse monoclonal [HL2010] to Chondroitinase ABC I -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment within Chondroitinase ABC I aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Proteus vulgaris.
Database link: P59807 -
Positive control
- WB: Proteus vulgaris Chondroitinase ABC recombinant Protein (aa25-1021).
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL2010 -
Isotype
IgG1
Images
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Anti-Chondroitinase ABC I antibody [HL2010] (ab256485) at 0.092 µg/ml + Proteus vulgaris Chondroitinase ABC recombinant Protein (aa25-1021), 20 ng
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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ELISA - Anti-Chondroitinase ABC I antibody [HL2010] (ab256485).
Antigen: Chondroitinase ABC at 1000 ng/ml.
ab256485 used at 20-5000 ng/ml.
Secondary is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at 1/1000 dilution.
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