Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y]
See all Ionotropic glutamate receptor 2 + 3 primary antibodies -
Description
Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3 -
Host species
Rabbit -
Specificity
ab52896 detects both GluR2 + 3 phosphorylated on Serine 880/891 of the mature human, and also unphosphorylated GluR2 + 3. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ionotropic glutamate receptor 2 + 3 (phospho S880 + S891). The exact sequence is proprietary.
(Peptide available asab205220) -
Positive control
- WB: Human brain and fetal tissue lysates and mouse and rat brain tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP929Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/10000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/5000 dilution (unpurified) + Human brain tissue lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 98 kDa
Observed band size: 98 kDa
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