Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GATA2 antibody [EPR2822(2)] (ab109241)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2822(2)] to GATA2
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GATA2 antibody [EPR2822(2)]
    See all GATA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2822(2)] to GATA2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: bEnd.3, PC-12, HEK-293, and K562 whole cell lysates. Mouse placenta lysate; IP: K-562 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] (ab109241)
    Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] (ab109241)
    GATA2 was immunoprecipitated from 0.35 mg K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate 10 µg with ab109241 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab109241 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate 10 µg

    Lane 2: ab109241 IP in K-562 cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109241 in K562 cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds

  • Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)
    Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)
    All lanes : Anti-GATA2 antibody [EPR2822(2)] (ab109241) at 1 µg/ml

    Lane 1 : Wild-type HEK-293 whole cell lysate
    Lane 2 : GATA2 knockout HEK-293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 51 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab109241 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109241 was shown to recognize GATA2 in wild-type HEK-293 cells as signal was lost at the expected MW in GATA2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GATA2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab109241 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] (ab109241)
    Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] (ab109241)

    Purified ab109241 at 1/30 dilution (2 µg) immunoprecipitating GATA2 in K-562 whole cell lysate.
    Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg
    Lane 2 (+): ab109241 + K-562 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109241 in K-562 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 51 kDa

  • Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)
    Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)
    All lanes : Anti-GATA2 antibody [EPR2822(2)] (ab109241) at 1/1000 dilution (purified)

    Lane 1 : bEnd.3 (Mouse brain endothelioma) whole cell lysate
    Lane 2 : Mouse placenta lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)
    Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)
    All lanes : Anti-GATA2 antibody [EPR2822(2)] (ab109241) at 1/1000 dilution

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate prepared using 1% SDS hot lysis method
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate prepared using RIPA lysis method
    Lane 3 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST.

    The different result in K-562 is due to the lysates preparation method.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    The expression profile observed in U-937 is consistent with the literature (PMID: 19212333).
    Negative control: U-937 (PMID: 19212333)

  • Anti-GATA2 antibody [EPR2822(2)] (ab109241)
    Anti-GATA2 antibody [EPR2822(2)] (ab109241)

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