Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (ab239959)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2822(2)] to GATA2 - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free
    See all GATA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2822(2)] to GATA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: bEnd.3, PC-12, HEK-293, and K562 whole cell lysates. Mouse placenta lysate; IP: K-562 whole cell lysate.

  • General notes

    Ab239959 is the carrier-free version of ab109241. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239959 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (ab239959)
    Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (ab239959)

    GATA2 was immunoprecipitated from 0.35 mg K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate 10 µg with ab109241 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab109241 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate 10 µg

    Lane 2: ab109241 IP in K-562 cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109241 in K562 cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109241).

  • Western blot - Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (ab239959)
    Western blot - Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (ab239959)
    All lanes : Anti-GATA2 antibody [EPR2822(2)] (ab109241) at 1/1000 dilution (purified)

    Lane 1 : bEnd.3 (Mouse brain endothelioma) whole cell lysate
    Lane 2 : Mouse placenta lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109241).

  • Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (ab239959)
    Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (ab239959)

    Purified ab109241 at 1/30 dilution (2 µg) immunoprecipitating GATA2 in K-562 whole cell lysate.
    Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg
    Lane 2 (+): ab109241 + K-562 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109241 in K-562 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 51 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109241).

  • Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (ab239959)
    Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (ab239959)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (ab239959)

© 2021 Astana Biomed Group LLP. All rights reserved.