Anti-RIL antibody (ab90157)
Key features and details
- Rabbit polyclonal to RIL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RIL antibody
See all RIL primary antibodies -
Description
Rabbit polyclonal to RIL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human RIL aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab95864) -
Positive control
- This antibody gave a positive signal in A431 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatRabbitChickenCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).Target
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Tissue specificity
Isoform 2 is found in brain. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsPhosphorylated on tyrosine residue(s). Can be dephosphorylated by PTPN13. - Information by UniProt
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Database links
- Entrez Gene: 395643 Chicken
- Entrez Gene: 515410 Cow
- Entrez Gene: 8572 Human
- Entrez Gene: 30794 Mouse
- Entrez Gene: 24915 Rat
- Omim: 603422 Human
- SwissProt: Q9PW72 Chicken
- SwissProt: Q3T005 Cow
see all -
Alternative names
- Enigma homolog antibody
- LIM domain protein antibody
- LIM domain protein RIL antibody
see all
Images
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Western blot - Anti-RIL antibody (ab90157)Anti-RIL antibody (ab90157) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
RIL contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab90157 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-RIL antibody (ab90157)Anti-RIL antibody (ab90157) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
RIL contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
