Pig Haptoglobin ELISA Kit (ab205091)
Key features and details
- Sensitivity: 3.107 ng/ml
- Range: 6.25 ng/ml - 400 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Pig Haptoglobin ELISA Kit
See all Haptoglobin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum Inter-assay Sample n Mean SD CV% Serum -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.107 ng/ml -
Range
6.25 ng/ml - 400 ng/ml -
Recovery
> 85 %
Sample specific recovery Sample type Average % Range Serum > 85 6.25ng/ml - 400ng/ml -
Assay time
0h 40m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
The Pig Haptoglobin (ab205091) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Haptoglobin in biological fluid of pigs.
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Notes
In this assay the Haptoglobin present in samples reacts with the anti-Haptoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hp in the test sample. The quantity of Hp in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP Conjugated Enzyme Antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentration 1 x 50ml Anti-Pig Haptoglobin ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Pig Hapatoglobin Calibrator (lyophilized) 1 vial Stop Solution 1 x 12ml -
Research areas
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Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Anhaptoglobinemia -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Binding peptide
- BP
- Haptoglobin alpha chain
see all
Images
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Standard Curve
Representative standard curve using ab205091 Pig Haptoglobin ELISA Kit.
