Swine Haptoglobin ELISA Kit (ab223136)
Key features and details
- Sensitivity: 0.55 ng/ml
- Range: 1.875 ng/ml - 120 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Swine Haptoglobin ELISA Kit
See all Haptoglobin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 1 20 5.4% Plasma 2 20 4.9% Plasma 3 20 5.6% Inter-assay Sample n Mean SD CV% Plasma 1 20 9.9% Plasma 2 20 9.5% Plasma 3 20 10.1% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.55 ng/ml -
Range
1.875 ng/ml - 120 ng/ml -
Recovery
97 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig
Does not react with: Mouse, Rat, Rabbit, Cow, Human, Monkey -
Product overview
The Swine Haptoglobin ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab137979) is designed for detection of swine haptoglobin in plasma, serum, urine, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures swine haptoglobin in approximately 4 hours. A polyclonal antibody specific for swine haptoglobin has been pre-coated onto a 96-well microplate with removable strips. Swine haptoglobin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for swine haptoglobin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Haptoglobin (HP, zonulin) is a plasma protein with hemoglobin-binding capacity and a plasma glycoprotein that forms a stable complex with hemoglobin to aid the recycling of heme iron.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 80X Biotinylated Swine Haptoglobin Antibody 1 x 75µl Anti-Swine Haptoglobin coated Microplate (12 x 8 wells) 1 unit Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Swine Haptoglobin Standard 1 vial -
Research areas
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Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Anhaptoglobinemia -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Binding peptide
- BP
- Haptoglobin alpha chain
see all
Images
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Swine Haptoglobin Standard Curve Example
Example of Swine Haptoglobin standard curve in 1X Diluent N. Background-subtracted data values (mean +/- SD) are graphed.
