Anti-Chk2 (phospho T383) antibody (ab59408)
Key features and details
- Rabbit polyclonal to Chk2 (phospho T383)
- Suitable for: ICC, WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Chk2 (phospho T383) antibody
See all Chk2 primary antibodies -
Description
Rabbit polyclonal to Chk2 (phospho T383) -
Host species
Rabbit -
Specificity
Detects Human Chk2 only when phosphorylated at threonine 383 (Mouse: Thr387; Rat: Thr386) -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Human Chk2.
Database link: O96017 -
Positive control
- WB: COS7 cell extracts, UV treated (30 mins). ICC: Human colon carcinoma cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Chk2 (phospho T383) antibody (ab59408) at 1/500 dilution
Lane 1 : COS7 cell extracts, UV treated (30 mins)
Lane 2 : COS7 cell extracts, UV treated (30 mins) with immunising phosphopeptide
Predicted band size: 61 kDa
Observed band size: 61 kDa
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Chk2 (phospho T383) (green) using ab59408 at 1/100 dilution in ICC/IF, in the presence (right panel) or absence (left panel) of immunizing phosphopetide.
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ab59408 staining Chk2 (phospho T383) in human colon carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 37°C. A FITC-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.