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Anti-Chk2 (phospho T383) antibody (ab59408)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Chk2 (phospho T383) antibody (ab59408)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Chk2 (phospho T383)
  • Suitable for: ICC, WB
  • Reacts with: Human, African green monkey
  • Isotype: IgG

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Overview

  • Product name

    Anti-Chk2 (phospho T383) antibody
    See all Chk2 primary antibodies
  • Description

    Rabbit polyclonal to Chk2 (phospho T383)
  • Host species

    Rabbit
  • Specificity

    Detects Human Chk2 only when phosphorylated at threonine 383 (Mouse: Thr387; Rat: Thr386)
  • Tested applications

    Suitable for: ICC, WBmore details
  • Species reactivity

    Reacts with: Human, African green monkey
  • Immunogen

    Synthetic peptide corresponding to Human Chk2.
    Database link: O96017

  • Positive control

    • WB: COS7 cell extracts, UV treated (30 mins). ICC: Human colon carcinoma cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Chk1 / Chk2
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other

Images

  • Western blot - Anti-Chk2 (phospho T383) antibody (ab59408)
    Western blot - Anti-Chk2 (phospho T383) antibody (ab59408)
    All lanes : Anti-Chk2 (phospho T383) antibody (ab59408) at 1/500 dilution

    Lane 1 : COS7 cell extracts, UV treated (30 mins)
    Lane 2 : COS7 cell extracts, UV treated (30 mins) with immunising phosphopeptide

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

  • Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (ab59408)
    Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (ab59408)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Chk2 (phospho T383) (green) using ab59408 at 1/100 dilution in ICC/IF, in the presence (right panel) or absence (left panel) of immunizing phosphopetide.

  • Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (ab59408)
    Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (ab59408) This image is courtesy of an Abreview submitted by Eleni Petsalaki

    ab59408 staining Chk2 (phospho T383) in human colon carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 37°C. A FITC-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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