Anti-LAT (phospho Y171) antibody (ab73205)
Key features and details
- Rabbit polyclonal to LAT (phospho Y171)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LAT (phospho Y171) antibody
See all LAT primary antibodies -
Description
Rabbit polyclonal to LAT (phospho Y171) -
Host species
Rabbit -
Specificity
ab73205 detects endogenous levels of LAT only when phosphorylated at tyrosine 171. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human LAT around the phosphorylation site of tyrosine 171 (DDYPVN)
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Positive control
- Extracts from Jurkat cells treated with UV (5mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab73205 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LAT (phospho Y171) antibody (ab73205)All lanes : Anti-LAT (phospho Y171) antibody (ab73205) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells treated with UV (5 min)
Lane 2 : Extracts from Jurkat cells treated with UV (5 min) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
