Anti-LAT (phospho Y191) antibody (ab59197)
Key features and details
- Rabbit polyclonal to LAT (phospho Y191)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-LAT (phospho Y191) antibody
See all LAT primary antibodies -
Description
Rabbit polyclonal to LAT (phospho Y191) -
Host species
Rabbit -
Specificity
Detects endogenous levels of LAT only when phosphorylated at tyrosine 191. This antibody detects LAT phosphorylated on Tyrosine 191 in isoform 2 (SwissProt: O43561-2), and Tyrosine 220 in isoform 1 (SwissProt: O43561). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic phosphopeptide from around the phosphorylation site of tyrosine 191 (REYPVN) of human LAT
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT (phospho Y191) antibody (ab59197)Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59197 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.
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Western blot - Anti-LAT (phospho Y191) antibody (ab59197)All lanes : Anti-LAT (phospho Y191) antibody (ab59197) at 1/500 dilution
Lane 1 : NIH3T3 cells treated with PMA
Lane 2 : NIH3T3 cells treated with PMA with phosphopeptide
Predicted band size: 28 kDa
Observed band size: 28 kDa
