Anti-LAT antibody [Y109] (ab32070)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y109] to LAT
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-LAT antibody [Y109]
See all LAT primary antibodies -
Description
Rabbit monoclonal [Y109] to LAT -
Host species
Rabbit -
Specificity
This antibody is specific for LAT. It detects the long and short forms of LAT. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human LAT aa 200-300. The exact sequence is proprietary. Corresponds to residues surrounding the GRB2 binding motif 2.
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Positive control
- WB: Jurkat whole cell lysate (ab7899). IHC-P: Human lymph node. Flow Cyt: Jurkat cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
Y109 -
Isotype
IgG -
Research areas
Images
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Anti-LAT antibody [Y109] (ab32070) at 1/500 dilution + Jurkat cell lysate.
Predicted band size: 28 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
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Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling LAT with purified ab32070 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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Ab32070, at a dilution of 1/100, staining LAT in paraffin embedded human lymph node tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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